SDS PAGE - (Sodium dodecyl Sulfate - ployacrylamide gel electrophoresis) is a method for protein separation based on mass is an electrophoretic technique. The polyacrilamide is poured onto the gel slabs for the medium of electric field, and that is discontinuous and sandwiched between two glass plates. SDS is a surfactant changing the proteins charge that helps the migration of proteins to the anode based on the charge applied by the electric field across the gel slabs. SDS is amphipathic for folding of polar and no polar sections. Gradient gels of acrylamide have larger separation ranges of molecular masses. The denatured samples are loaded onto gel polyacrylamide which are placed along with suitable electrophoresis and electrolytes. Negatively charged molecules move toward the positive side, with larger molecules retained and smaller moving faster, thus, separating the molecules by their molecular sizes. The bromophenol blue in the sample makes their migration visible. Due to the relative smaller size, this chemical moves faster than the proteins. The individual molecular weights deter,Paine the protein bands in the separating gel. The relative distances over the size of the proteins as marker are plotted semi-logarithmically against their own molecular weights. It is easy to use, quick for analytical purposes. This method is used with the combination of western blot for the Presence of proteins and for the analysis of post translational modifications.
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