Fatty acids are one of the four building blocks of biological systems. They are made of varied fatty acids differing in chain length and unsaturation along with double bonds. Deficiencies and excess fatty acids in body results in different types of diseases. Various analytical techniques for fatty acids in various biological samples. Having the best separation capability is necessary to produce an authentic and reliable GC MS platform for fatty acid analysis. In indirect method they were converted to corresponding methyl esters (FAMEs) during trimethylsulfonium hydroxide solution reaction. The peaks are obtained based on their fractionation. This is used for understanding the fatty acid structures, functions, anti microbial activities etc.
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