Protein sequencing is to understand the amino acid sequence of a protein along with its structure and function. Amino acid sequence also determines the three dimensional structure of the protein. All proteins of polymers have amino acids accepting two or one amino group attached to the carboxyl group. The intact proteins are introduced into mass analyser. In a process, the proteins are divided into fragments or peptides and introduced into mass analyser. Protein sequencing is involved in protein sequencing, denaturation of proteins, alkylate disulfide, identifying subunits, cleavage and edman degradation. Mass spectrometry is an important method for the characterization of proteins by two primary methods for ionization of whole proteins are electrospray ionization and MALDI. Mass spectrometry has three main parts for characterizing protein and ionization sources, a mass analyzer, an ion detector.
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