Fluorescence is an emission of energy, mainly electromagnetic radiation, transition from a higher to lower state within the molecule’s sub atomic states, measured by emitted radiation rather than the absorption. A molecule absorbs light at one wavelength and remits light as a longer wavelength. The basic principle of fluorescens of luminescence is emission of photons from electrically excited states. Fluorescence is a highly sensitive and accurate technique. This method is used for quantitative analysis in the chemical, biological sciences, analytical chemistry, biochemistry, pharmacy and medicinal industry to detect compounds from HPLC flow, plants pigments, steroids, proteins, naphthols.
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