iTRAQ is used in quantitative proteomics by mass spectrometry as an isobaric labelling process in tandem mass spectrometry. The proteins from various sources are taken in a single experiment based on their masses, are labelled with Isobaric tags for its proportional and absolute quantitation. It uses the labelling method of molecules based on vpcovalnet labelling at the N-terminus end of the protein that are covalently bonded to its side chain amines of proteins. They are tagged with isobaric tags from tags of various protein masses. Examples for labelling substances are 4-plex and 8-plex for determining through mass spectrometry. Later samples are pooled by fractionation of liquid chromatography. Objectives of developing iTRAQ of many differential effects on proteins themselves come from posttranslational modification. Most common isobaric tag is an amine reactive. Factors affecting on iTRAQ evaliton of labelling efficiency and isotope impurity correction, ratio compression and its correction, reporter ion intensity dynamic range.
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