Mate pair sequencing involves a paired end reads with long inserts and this long insert paired end is called mate pair. c DNA is first
fragmented to reduce the high molecular weight DNA into smaller fragments
of a desired size range which are end-repaired and
biotin-labeled at the ends. Then, DNA fragments of a particular size range are selected based on gap
size variance, of the paired reads and circularized by an intramolecular ligation.
All the linear DNA are removed by exonuclease, then, sheared again either by Covaris shearing or
nebulization and are purified using streptavidin-coated magnetic beads and further end-repaired and
A-tailed by illumina paired-end oligo adapters. This is generating long insert paired end DNA libraries by PCR of 300-500 cycles, useful for number of sequencing applications including De Novo Sequencing structural variant detection Genome finishing and identification of complex genomic rearrangements for cluster generation and sequencing. There are two kinds of reads with paired end sequencing short insert paired end reads and long insert paired end reads. when combining data generated from mate pair library sequencing with that from short insert paired ends reads which provides a powerful combination of the read length for the maximal sequencing across the genome.
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