It is a method that is used to analyze the protein interactions with the help of Dna. It identifies the Dna binding sites with their respective associated proteins by combining with the chromatin immunoprecipitation with the Dna sequencing. This is used to map the global sequencing sites of any protein by determining the transcription factors and other proteins associated to the chromatin influencing the phenotypes of the mechanism. This can be applied to Chip able proteins and modifications of transcription factors, polymerases, transcription machinery, structural proteins, protein and DNA modifications. It is used to determine that how proteins interacts with Dna that regulate the gene expression that id essential in the biological process and disease state. Usually it starts with the crosslinking of Dna protein complexes. The samples are then fragmented and it treated with an exonuclease to trim unbound oligonucleotides. These oligonucleotides are are added to the small stretches that were bound to the protein of interest to enable massively parallel sequencing.
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