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Delve into the practical applications of microbiology with our Applied Microbiology Research Training, from industrial processes to environmental solutions.
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NTHRYS provides Applied Microbiology Research Training Program at its Hyderabad facility, Telangana.
Please refer below for more details including Fee strctures,
Eligibility, Protocols and Modules etc.,. Please do call / message / whatsapp for more details on +91-7993084748.
Eligibility: BSc / BTech / MSc / MTech / MPhil / PhD in relevant field studying or completed students.
Module 1: Environmental Applied Microbiology Techniques
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Profits of Learning Environmental Applied Microbiology Techniques
Learning environmental applied microbiology techniques is essential for addressing environmental challenges such as pollution, waste management, and ecosystem health. These protocols provide insights into microbial roles in biogeochemical cycles, biodegradation, and bioremediation. Mastery of these techniques enables researchers to develop sustainable solutions for environmental conservation, enhance the understanding of microbial ecology, and contribute to environmental biotechnology advancements.
Environmental Level Approaches and Protocols
- Water Quality Testing (pH Meter, DO Meter, Conductivity Meter)
- Soil Microbial Analysis (Soil Samplers, Culture Media)
- Air Sampling Techniques (Air Samplers, Agar Plates)
- Microbial Source Tracking (PCR, Gel Electrophoresis)
- Microbial Diversity Analysis (Next-Generation Sequencing, Bioinformatics Tools)
- Biogeochemical Cycling Assays (Gas Chromatograph, Isotope Ratio Mass Spectrometer)
- Pathogen Detection in Water (qPCR, ELISA Kits)
- Microbial Community Profiling (DGGE, Metagenomics)
- Microbial Contaminant Degradation (Bioreactors, Analytical Instruments)
- Microbial Ecology Studies (Metagenomics, Fluorescence Microscopy)
- Microbial Symbiosis Assays (Co-culture Systems, Microscopy)
- Environmental DNA (eDNA) Extraction (Centrifuge, DNA Purification Kits)
- Bioindicator Analysis (Bioassays, Spectrophotometer)
- Microbial Phytoremediation (Plant-Microbe Interaction Assays, Growth Chambers)
- Microbial Soil Health Indicators (Soil Respiration Assays, Microbial Biomass)
- Pollutant Biodegradation Studies (Bioreactors, Analytical Chemistry Techniques)
- Bioaerosol Monitoring (Air Samplers, PCR)
- Marine Microbiology Techniques (Marine Culture Media, Incubators)
- Wastewater Microbiology (Activated Sludge Units, Microscopy)
- Microbial Bioreactors for Waste Treatment (Bioreactors, Gas Chromatograph)
- Microbial Consortia for Biodegradation (Bioreactors, HPLC)
- Heavy Metal Bioremediation (Bioreactors, Atomic Absorption Spectroscopy)
- Oil Spill Bioremediation (Bioreactors, Hydrocarbon Analysis)
- Microbial Electrosynthesis (Microbial Electrolysis Cells, Power Meters)
- Biochar Production and Applications (Pyrolysis Units, Soil Analysis)
- Microbial Carbon Sequestration (Biochar, Soil Microbial Analysis)
- Ecological Engineering (Constructed Wetlands, Ecosystem Monitoring)
- Microbial Treatment of Landfill Leachate (Bioreactors, Analytical Instruments)
- Biogas Production from Organic Waste (Anaerobic Digesters, Gas Chromatograph)
- Microbial Mining Techniques (Bioleaching, Bioreactors)
- Biological Nutrient Removal in Wastewater (Denitrification, Phosphorus Removal)
- Development of Eco-Friendly Pesticides (Microbial Formulations, Field Trials)
- Waste-to-Resource Technologies (Fermentation, Composting)
- Biodegradable Plastics Production (Fermentation, Polymerization)
- Microbial Ecology of Extreme Environments (Sampling Techniques, Metagenomics)
Duration: 3 Months
Fee: Rs 2,50,000/-
Statutory Note: NTHRYS Team can change the protocols, software, or tools used to achieve the tasks linked to the above-mentioned approaches or protocols.
Module 2: Clinical Applied Microbiology Techniques
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Profits of Learning Clinical Applied Microbiology Techniques
Mastering clinical applied microbiology techniques is crucial for diagnosing infectious diseases, understanding pathogen biology, and developing treatments. These protocols enable researchers to identify, culture, and study microorganisms that cause diseases, ensuring accurate diagnosis and effective treatment strategies. Proficiency in these techniques supports advancements in medical research, public health, and clinical microbiology.
Clinical Level Approaches and Protocols
- Blood Culture Techniques (Automated Blood Culture Systems)
- Urine Culture Methods (Cystine Lactose Electrolyte Deficient Agar, Chromogenic Media)
- Sputum Culture Techniques (Lowenstein-Jensen Medium, Chocolate Agar)
- Throat Swab Culture (Blood Agar, Selective Media)
- Wound Swab Culture (MacConkey Agar, Nutrient Agar)
- Stool Culture Techniques (XLD Agar, SS Agar)
- Antimicrobial Susceptibility Testing (Kirby-Bauer Disk Diffusion, E-test)
- Rapid Diagnostic Tests (Lateral Flow Assays, PCR)
- Serological Testing (ELISA, Western Blot)
- Mycobacterium Tuberculosis Detection (Ziehl-Neelsen Staining, GeneXpert)
- Identification of Enteric Pathogens (API 20E, Enterotube)
- Virology Techniques (Cell Culture, Plaque Assay)
- Parasitology Methods (Concentration Techniques, Microscopy)
- Fungal Culture Techniques (Sabouraud Dextrose Agar, Cornmeal Agar)
- Molecular Diagnostics (qPCR, Sequencing)
- Antibiotic Resistance Detection (MIC Determination, Genotypic Methods)
- Nosocomial Infection Surveillance (Swab Sampling, Environmental Monitoring)
- Microbial Genotyping (PFGE, MLST)
- Point-of-Care Testing (Rapid Tests, Portable Analyzers)
- Automated Identification Systems (VITEK, MALDI-TOF)
- Biofilm Formation Assays (Microtiter Plates, Crystal Violet Staining)
- Clinical Specimen Handling (Biosafety Cabinets, Sterile Techniques)
- Infection Control Protocols (Hand Hygiene, Disinfection)
- Monitoring of Antimicrobial Stewardship (Antibiotic Usage Audits)
- Microbial Load Assessment in Clinical Settings (Air Samplers, Surface Swabs)
- Emerging Pathogen Detection
Duration: 2 Months
Fee: Rs 1,20,000/-
Statutory Note: NTHRYS Team can change the protocols, software, or tools used to achieve the tasks linked to the above-mentioned approaches or protocols.
Module 3: Applied Microbial Genetics Techniques
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Profits of Learning Applied Microbial Genetics Techniques
Learning applied microbial genetics techniques is essential for manipulating microbial genomes to study gene function, regulation, and expression. These protocols enable researchers to understand genetic pathways, develop genetically modified organisms, and explore microbial diversity at the genetic level. Mastery of these techniques supports advancements in biotechnology, medicine, and microbial ecology, contributing to innovative research and applications.
Genetics Level Approaches and Protocols
- Gene Cloning Techniques (PCR, Restriction Digestion, Ligation)
- Genetic Transformation of Microbes (Electroporation, Heat Shock)
- Recombinant Protein Expression (Expression Vectors, Induction Conditions)
- Plasmid Isolation (Alkaline Lysis, Plasmid Purification Kits)
- Southern Blotting (DNA Probes, Hybridization Ovens)
- Northern Blotting (RNA Probes, Electrophoresis)
- Western Blotting (Antibodies, Blotting Membranes)
- RNA Interference (siRNA, shRNA)
- Reporter Gene Assays (Luciferase, GFP)
- Transposon Mutagenesis (Transposon Libraries, Screening)
- Gene Synthesis and Assembly (Synthetic Biology Tools)
- Microbial Genomics (Genome Sequencing, Annotation)
- Microbial Functional Genomics (Gene Function Studies)
- Metagenomics (Metagenomic Sequencing, Bioinformatics Tools)
- Transcriptome Analysis (RNA-Seq, Microarrays)
- Proteomics (Mass Spectrometry, 2D Gel Electrophoresis)
- Protein Purification (Affinity Chromatography, FPLC)
- Protein Quantification (Bradford Assay, BCA Assay)
- Gene Expression Analysis (qPCR, RNA-Seq)
- Bioinformatics Analysis (Computational Tools, Databases)
- High-Throughput Screening (Microtiter Plates, Robotics)
- Functional Genomics Studies (Gene Knockout, Overexpression)
- Comparative Genomics (Genome Alignment, Phylogenetics)
- Single-Cell Genomics (Microfluidics, Single-Cell Sequencers)
- Epigenetic Analysis (Bisulfite Sequencing, ChIP-Seq)
- Microbial Genotyping (PFGE, MLST)
- Yeast Two-Hybrid Screening (Yeast Strains, Reporter Assays)
- Phage Display (Phage Libraries, Screening)
- Microbial Genome Editing (CRISPR Tools, Gene Knockout)
- Comparative Transcriptomics (RNA-Seq, qRT-PCR)
- Genetic Mapping (Linkage Analysis, QTL Mapping)
- Microbial Population Genetics (Allele Frequency Analysis, Population Structure)
- Functional Annotation of Genes (Gene Ontology, KEGG Pathways)
- Genome-Wide Association Studies (GWAS) (Genotyping, Statistical Analysis)
- Microbial Strain Engineering (Synthetic Biology, Metabolic Engineering)
Duration: 3 Months
Fee: Rs 3,00,000/-
Statutory Note: NTHRYS Team can change the protocols, software, or tools used to achieve the tasks linked to the above-mentioned approaches or protocols.
Module 4: Applied Microbial Antigen Studies
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Profits of Learning Applied Microbial Antigen Studies
Learning applied microbial antigen studies is essential for developing vaccines, diagnostic tools, and immunotherapies. These protocols help researchers identify and characterize microbial antigens, leading to advancements in infectious disease management and immune response understanding. Mastery of these techniques supports innovations in microbiology, immunology, and biotechnology, contributing to public health improvements and medical advancements.
Antigen Studies Approaches and Protocols
- Isolation of Microbial Antigens (Centrifugation, Ultrafiltration)
- Characterization of Antigens (SDS-PAGE, Western Blotting)
- Antigen Purification (Affinity Chromatography, Gel Filtration)
- Preparation of Antigen Stocks (Lyophilization, Freezing)
- Detection of Antigens (ELISA, Immunofluorescence)
- Antigen-Antibody Reactions (Immunoprecipitation, Agglutination Tests)
- Identification of Antigenic Determinants (Epitope Mapping, Peptide Synthesis)
- Antigen Cloning and Expression (PCR, Recombinant DNA Techniques)
- Production of Polyclonal Antibodies (Animal Immunization, Serum Collection)
- Antigen-Antibody Binding Assays (Surface Plasmon Resonance, ITC)
- Antigen Stability Studies (Thermal Stability, Protease Resistance)
- Immunogenicity Testing (Animal Models, Cytokine Assays)
- Cross-Reactivity Studies (ELISA, Immunoblotting)
- Mass Spectrometry for Antigen Analysis (MALDI-TOF, ESI-MS)
- In Silico Antigen Prediction (Bioinformatics Tools)
- Recombinant Antigen Production (E. coli Expression Systems, Yeast Expression Systems)
- Conjugation of Antigens to Carriers (Protein Conjugation, Nanoparticle Conjugation)
- Development of Diagnostic Kits (Lateral Flow Assays, ELISA Kits)
- Antigen Storage and Preservation (Cryopreservation, Lyophilization)
- Immunoassay Development (Sandwich ELISA, Competitive ELISA)
- Assessment of Vaccine Efficacy (Neutralization Tests, Protection Studies)
- Quality Control of Antigen Preparations (Purity Analysis, Potency Testing)
- Validation of Immunoassays (Specificity, Sensitivity)
- Application of Antigens in Serological Surveys (Epidemiological Studies)
- Antigen Presentation Studies (MHC Binding Assays, T-Cell Activation Assays)
- Adjuvant Formulation for Vaccines (Emulsions, Particulates)
- Development of Subunit Vaccines (Protein Engineering, Formulation)
- Antigen-Antibody Interaction Studies (Kinetic Analysis, Binding Affinity)
Duration: 4 Months
Fee: Rs 4,50,000/-
Statutory Note: NTHRYS Team can change the protocols, software, or tools used to achieve the tasks linked to the above-mentioned approaches or protocols.
Module 5: Applied Microbial Genetics in Antibiotic Resistance
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Profits of Learning Applied Microbial Genetics in Antibiotic Resistance
Learning applied microbial genetics in antibiotic resistance is crucial for understanding the mechanisms behind resistance, tracking the spread of resistance genes, and developing new strategies to combat antibiotic-resistant infections. These protocols enable researchers to study genetic mutations, horizontal gene transfer, and the impact of environmental factors on resistance. Mastery of these techniques supports advancements in microbiology, public health, and pharmaceutical development, contributing to more effective treatments and containment strategies.
Antibiotic Resistance Genetics Approaches and Protocols
- Isolation of Antibiotic-Resistant Strains (Selective Media, Culture Techniques)
- Phenotypic Characterization of Resistance (MIC Determination, Disk Diffusion Assays)
- Genomic DNA Extraction (DNA Purification Kits, Centrifugation)
- Identification of Resistance Genes (PCR, Sequencing)
- Whole Genome Sequencing (NGS, Bioinformatics Tools)
- Plasmid Profiling (Agarose Gel Electrophoresis, Restriction Digestion)
- Conjugation Experiments (Mating Assays, Plasmid Transfer)
- Transformation and Transduction (Competent Cells, Bacteriophage Methods)
- Mutagenesis Studies (Chemical Mutagens, UV Radiation)
- Resistance Gene Cloning (Cloning Vectors, Expression Systems)
- Transcriptomic Analysis (RNA-Seq, qRT-PCR)
- Proteomic Profiling (Mass Spectrometry, 2D Gel Electrophoresis)
- Horizontal Gene Transfer Studies (Conjugation, Transformation)
- Antibiotic Resistance Mechanism Studies (Efflux Pumps, Target Modification)
- Functional Genomics (Gene Knockout, Overexpression)
- Resistance Gene Expression Analysis (qPCR, Reporter Assays)
- Microbial Fitness Assays (Growth Curves, Competition Assays)
- Bioinformatics Analysis of Resistance Genes (Databases, Computational Tools)
- Environmental Sampling for Resistance Genes (Soil, Water Sampling)
- Metagenomics of Resistomes (High-Throughput Sequencing, Data Analysis)
- Monitoring Antibiotic Resistance in Clinical Settings (Surveillance Studies, Epidemiology)
- Development of Diagnostic Tests for Resistance (PCR, Rapid Tests)
- Antibiotic Resistance Gene Detection (Multiplex PCR, Microarrays)
- Antibiotic Susceptibility Testing (E-test, Broth Microdilution)
- Mobile Genetic Elements Analysis (Transposons, Integrons)
- Comparative Genomics of Resistant Strains (Phylogenetics, SNP Analysis)
- Impact of Antibiotic Use on Resistance (Selective Pressure Studies, Longitudinal Studies)
- Resistance Gene Transfer in Biofilms (Biofilm Models, Microscopy)
- Regulation of Resistance Genes (Promoter Analysis, Transcription Factors)
- Quantitative PCR for Resistance Gene Quantification (qPCR, Standard Curves)
- Antibiotic Resistance Plasmid Stability (Plasmid Curing, Segregational Stability)
- Antibiotic Degradation Assays (Enzyme Activity, HPLC Analysis)
- Development of Novel Antibiotics (Screening Libraries, Activity Assays)
- Combination Therapy Studies (Synergy Testing, Checkerboard Assays)
- Resistance Gene Silencing (RNAi, CRISPRi)
- Antibiotic Resistance in Pathogenic vs. Commensal Bacteria (Comparative Studies)
Duration: 4 Months
Fee: Rs 3,50,000/-
Statutory Note: NTHRYS Team can change the protocols, software, or tools used to achieve the tasks linked to the above-mentioned approaches or protocols.
Testimonials
VB. Bhavana View on Google
I have completed my 6 month dissertation in NTHRYS biotech labs. The lab is adequately equipped with wonderful, attentive and receptive staff. It is a boon to the students venturing into research as well as to students who would like to garner lab exposure. I had a pleasant experience at NTHRYS thanks to Balaji S. Rao Sir for his constant support, mettle and knowledge. I would also like to give special regards to Zarin Mam for teaching me the concepts of bioinformatics with great ease and for helping me in every step of the way. I extend my gratitude to Vijaya Mam, and Sindhu Mam for helping me carry out the project smoothly.
Durba C Bhattacharjee View on Google
I have just completed hands on lab trainings at NTHRYS in biotechnology which includes microbiology, molecular and immunology and had gained really very good experience and confidence having good infra structures with the guidance of Sandhya Maam and Balaji Sir.
Recommending to any fresher of biotechnology or microbiology field who wants to be expert before joining to
related industry.
Razia View on Google
Best place to aquire and practice knowledge.you can start from zero but at the end of the internship you can actually get a job that is the kind of experience you get here.The support and encouragement from the faculty side is just unexplainable because they make you feel like family and teach you every bit of the experiment.I strongly recommend NTHRYS Biotech lab to all the students who want to excel in their career.
Srilatha View on Google
Nice place for hands on training
Nandupandu View on Google
Very good place for students to learn all the techniques
Sadnaax View on Google
I apprenticed in molecular biology and animal tissue culture, helped me a lot for my job applications. Sandhya and Balaji sir were very supportive, very helpful and guided me through every step meticulously. Helped me learn from the basics and helped a lot practically. The environment of the lab is very hygienic and friendly. I had a very good experience learning the modules. Would recommend
Shivika Sharma View on Google
I did an internship in NTHRYS under Balaji sir and Sandhya maam. It was a magnificent experience. As I got hands-on experience on practicals and I was also provided with protocols and I learned new techniques too.This intership will help me forge ahead in life. The staff is very supportive and humble with everyone. Both sir and maam helped me with my each and every doubts without hesitation.
Digvijay Singh Guleria View on Google
I went for 2 months for different training programs at NTHRYS Biotech, had a fun learning experience. Everything was hands-on training and well organised protocols. Thank you Balaji sir and Sandhya mam for this life time experience.
Anushka Saxena View on Google
I’m a biotechnology student from Dy patil University mumbai and I recently completed my 6 months dissertation project at Nthrys Biotech Labs in Hyderabad. I had a great experience and I would highly recommend this lab to other students as well .
The first thing that I appreciated about Nthrys Biotech Labs was the friendly and supportive environment. Balaji sir and the staff Ragini and Sandhya ma’am were always willing to help me and they were always patient with my questions.
I also felt like I was part of a team and that I was making a real contribution to the companys research.
I learned a lot during my dissertation at Nthrys Biotech Labs not only academically but also personally . I had the opportunity to work on a variety of projects, which gave me a broad exposure to the field of biotechnology. I also learned a lot about the research process and how to conduct experiments.
In addition to the technical skills that I learned, I also developed my soft skills during my internship. I learned how to communicate effectively, how to work independently, and how to work as part of a team.
Overall, I had a great experience at Nthrys Biotech Labs and I would highly recommend this company to other students.
Once again I would like to render a big thank you to Balaji Sir and Vijayalakshmi ma’am for imbibing with all the knowledge along with helping me publish my research paper as well and its all because of them I scored unbelievably well in my final semester.
Nithin Pariki View on Google
Lab equipment and protocols are good, it gives good hands on experience for freshers.
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