Basic Microbiology Training Program

Fundamentals of Microbiology

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• Protocol for Gram Staining and Simple Staining Techniques
• Protocol for Acid-Fast Staining in Bacterial Analysis
• Protocol for Spore Staining and Endospore Identification
• Protocol for Capsule Staining in Microbial Cells
• Procedure for Wet Mount Preparation for Live Microbes
• Protocol for Culturing Microorganisms and Observing Growth Phases
• Procedure for Isolating Pure Microbial Cultures
• Method for Determining Microbial Cell Morphology and Size
• Protocol for Biochemical Testing in Microbial Classification
• Procedure for Catalase and Oxidase Testing
• Protocol for Carbohydrate Fermentation Testing
• Protocol for Measuring Microbial Respiration Rates
• Protocol for Nitrate Reduction Test in Bacteria
• IMViC Tests for Enteric Bacteria Identification

Microbial Techniques and Safety

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• Protocol for Preparation and Sterilization of Culture Media
• Autoclaving Protocol for Sterilization
• Filtration Technique for Heat-Sensitive Media
• Method for Plating and Spreading Microbial Cultures
• Protocol for Streak Plate Technique for Isolation
• Pour Plate Method for Enumeration of Microbes
• Serial Dilution Technique for Microbial Counting
• Colony Morphology and Pigmentation Analysis
• Antibiotic Sensitivity Testing Protocol (Kirby-Bauer Method)
• Minimum Inhibitory Concentration (MIC) Determination
• Protocol for Disc Diffusion Method
• Protocol for Broth Dilution Antimicrobial Testing
• Protocol for Swab Collection and Transport of Microbial Samples
• Safety Guidelines for Handling Pathogenic Microorganisms

Introduction to Microbial Genomics

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• Protocol for Agarose Gel Electrophoresis of DNA
• Polymerase Chain Reaction (PCR) Protocol for Microbial Identification
• Basic Protocol for Sanger Sequencing of Microbial DNA
• Protocol for 16S rRNA Sequencing for Bacterial Identification
• Protocol for Quantitative PCR (qPCR) Analysis
• Bioinformatics Protocol for 16S Sequence Analysis
• Basic Protocol for Next-Generation Sequencing (NGS)
• Restriction Fragment Length Polymorphism (RFLP) Analysis
• Protocol for Genetic Variation Analysis in Microbial Populations
• Plasmid Extraction and Purification Protocol
• Transformation Protocol in Bacteria using Plasmids
• Basic CRISPR-Cas Protocol for Gene Editing in Microbes
• RNA Extraction and Reverse Transcription PCR (RT-PCR)
• Protocol for Gene Expression Analysis in Microbes

Environmental Microbiology Basics

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• Membrane Filtration Method for Water Testing
• MPN Method for Coliform Detection in Water
• Soil Sample Collection and Microbial Analysis Protocol
• Protocol for Soil Enrichment Cultures for Specific Microbes
• Protocol for Analyzing Microbial Bioremediation Capabilities
• Hydrocarbon Degradation Assay for Soil Microbes
• Protocol for Biofilm Formation and Analysis
• Protocol for Testing Heavy Metal Resistance in Microbes
• Protocol for Detection of Environmental Pathogens
• Nitrogen Fixation Test in Soil Microbes
• Phosphate Solubilization Test in Soil Bacteria
• Protocol for Isolation of Rhizosphere Microbes
• Test for Organic Matter Decomposition by Soil Microbes
• Protocol for Microbial Community Analysis in Environmental Samples

Medical Microbiology Essentials

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• Procedure for Antimicrobial Susceptibility Testing
• Hemolysis Test on Blood Agar for Pathogenicity
• Protocol for Basic ELISA to Detect Microbial Antigens
• Procedure for Coagulase Test for Staphylococci
• Oxidase and Catalase Tests for Pathogen Identification
• Protocol for Latex Agglutination Test for Pathogen Detection
• Host-Pathogen Interaction Assay Protocol
• Vaccine Preparation and Testing in Model Organisms
• Protocol for Serological Testing in Infectious Diseases
• Molecular Identification of Pathogens by PCR
• Protocol for Immunofluorescence Assay
• Preparation of Cell Culture Media for Pathogen Growth
• Procedure for Detecting Fungal Pathogens in Clinical Samples
• Protocol for Virus Cultivation in Embryonated Eggs

Basic Immunology and Host Response

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• Flow Cytometry Protocol for Immune Cell Analysis
• ELISA Protocol for Detecting Host Antibodies
• Western Blot Protocol for Protein Detection in Immune Responses
• Protocol for Detecting Cytokines in Immune Response
• In Vitro Assay for T-Cell Activation
• In Vivo Assay for Measuring Immune Response to Vaccines
• Macrophage Phagocytosis Assay Protocol
• Protocol for Antigen-Antibody Agglutination Reactions
• Cell-Mediated Immunity Assay Protocol

Agricultural Microbiology Basics

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• Method for Isolation of Nitrogen-Fixing Bacteria
• Test for Plant Growth-Promoting Rhizobacteria (PGPR)
• Protocol for Biofertilizer Preparation Using Soil Microbes
• Production of Biopesticides Using Bacillus Thuringiensis
• Protocol for Plant-Microbe Interaction Studies
• Isolation of Endophytes from Plant Tissues
• Screening of Soil Microbes for Enzyme Production
• Protocol for Microbial Inoculant Production for Agriculture
• Test for Microbial Degradation of Agricultural Pesticides

Virology (Microbial Virology)

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• Plaque Assay for Quantification of Viruses
• Protocol for Viral RNA Extraction and Purification
• PCR and RT-PCR for Viral Genome Detection
• Protocol for Electron Microscopy Preparation of Viruses
• Viral Hemagglutination Assay Protocol
• Antiviral Drug Testing Protocol in Cell Cultures
• Procedure for Bacteriophage Therapy Assays
• Protocol for Vaccine Efficacy Testing in Virus Models
• Serological Testing Protocol for Viral Infections

Individual Protocols Under Basic Microbiology Training Program

1. Microscopy Protocol for Observing Microbial Cell Structure | Fee: Contact for fee
2. Protocol for Gram Staining and Simple Staining Techniques | Fee: Contact for fee
3. Protocol for Acid-Fast Staining in Bacterial Analysis | Fee: Contact for fee
4. Protocol for Spore Staining and Endospore Identification | Fee: Contact for fee
5. Protocol for Capsule Staining in Microbial Cells | Fee: Contact for fee
6. Procedure for Wet Mount Preparation for Live Microbes | Fee: Contact for fee
7. Protocol for Culturing Microorganisms and Observing Growth Phases | Fee: Contact for fee
8. Procedure for Isolating Pure Microbial Cultures | Fee: Contact for fee
9. Method for Determining Microbial Cell Morphology and Size | Fee: Contact for fee
10. Protocol for Biochemical Testing in Microbial Classification | Fee: Contact for fee
11. Procedure for Catalase and Oxidase Testing | Fee: Contact for fee
12. Protocol for Carbohydrate Fermentation Testing | Fee: Contact for fee
13. Protocol for Measuring Microbial Respiration Rates | Fee: Contact for fee
14. Protocol for Nitrate Reduction Test in Bacteria | Fee: Contact for fee
15. IMViC Tests for Enteric Bacteria Identification | Fee: Contact for fee
16. Aseptic Technique for Microbial Culture Handling | Fee: Contact for fee
17. Protocol for Preparation and Sterilization of Culture Media | Fee: Contact for fee
18. Autoclaving Protocol for Sterilization | Fee: Contact for fee
19. Filtration Technique for Heat-Sensitive Media | Fee: Contact for fee
20. Method for Plating and Spreading Microbial Cultures | Fee: Contact for fee
21. Protocol for Streak Plate Technique for Isolation | Fee: Contact for fee
22. Pour Plate Method for Enumeration of Microbes | Fee: Contact for fee
23. Serial Dilution Technique for Microbial Counting | Fee: Contact for fee
24. Colony Morphology and Pigmentation Analysis | Fee: Contact for fee
25. Antibiotic Sensitivity Testing Protocol (Kirby-Bauer Method) | Fee: Contact for fee
26. Minimum Inhibitory Concentration (MIC) Determination | Fee: Contact for fee
27. Protocol for Disc Diffusion Method | Fee: Contact for fee
28. Protocol for Broth Dilution Antimicrobial Testing | Fee: Contact for fee
29. Protocol for Swab Collection and Transport of Microbial Samples | Fee: Contact for fee
30. Safety Guidelines for Handling Pathogenic Microorganisms | Fee: Contact for fee
31. DNA Extraction and Purification Protocol for Microbial Samples | Fee: Contact for fee
32. Protocol for Agarose Gel Electrophoresis of DNA | Fee: Contact for fee
33. Polymerase Chain Reaction (PCR) Protocol for Microbial Identification | Fee: Contact for fee
34. Basic Protocol for Sanger Sequencing of Microbial DNA | Fee: Contact for fee
35. Protocol for 16S rRNA Sequencing for Bacterial Identification | Fee: Contact for fee
36. Protocol for Quantitative PCR (qPCR) Analysis | Fee: Contact for fee
37. Bioinformatics Protocol for 16S Sequence Analysis | Fee: Contact for fee
38. Basic Protocol for Next-Generation Sequencing (NGS) | Fee: Contact for fee
39. Restriction Fragment Length Polymorphism (RFLP) Analysis | Fee: Contact for fee
40. Protocol for Genetic Variation Analysis in Microbial Populations | Fee: Contact for fee
41. Plasmid Extraction and Purification Protocol | Fee: Contact for fee
42. Transformation Protocol in Bacteria using Plasmids | Fee: Contact for fee
43. Basic CRISPR-Cas Protocol for Gene Editing in Microbes | Fee: Contact for fee
44. RNA Extraction and Reverse Transcription PCR (RT-PCR) | Fee: Contact for fee
45. Protocol for Gene Expression Analysis in Microbes | Fee: Contact for fee
46. Protocol for Testing Microbial Contamination in Water Samples | Fee: Contact for fee
47. Membrane Filtration Method for Water Testing | Fee: Contact for fee
48. MPN Method for Coliform Detection in Water | Fee: Contact for fee
49. Soil Sample Collection and Microbial Analysis Protocol | Fee: Contact for fee
50. Protocol for Soil Enrichment Cultures for Specific Microbes | Fee: Contact for fee
51. Protocol for Analyzing Microbial Bioremediation Capabilities | Fee: Contact for fee
52. Hydrocarbon Degradation Assay for Soil Microbes | Fee: Contact for fee
53. Protocol for Biofilm Formation and Analysis | Fee: Contact for fee
54. Protocol for Testing Heavy Metal Resistance in Microbes | Fee: Contact for fee
55. Protocol for Detection of Environmental Pathogens | Fee: Contact for fee
56. Nitrogen Fixation Test in Soil Microbes | Fee: Contact for fee
57. Phosphate Solubilization Test in Soil Bacteria | Fee: Contact for fee
58. Protocol for Isolation of Rhizosphere Microbes | Fee: Contact for fee
59. Test for Organic Matter Decomposition by Soil Microbes | Fee: Contact for fee
60. Protocol for Microbial Community Analysis in Environmental Samples | Fee: Contact for fee
61. Protocol for Pathogen Detection using Rapid Diagnostic Tests | Fee: Contact for fee
62. Procedure for Antimicrobial Susceptibility Testing | Fee: Contact for fee
63. Hemolysis Test on Blood Agar for Pathogenicity | Fee: Contact for fee
64. Protocol for Basic ELISA to Detect Microbial Antigens | Fee: Contact for fee
65. Procedure for Coagulase Test for Staphylococci | Fee: Contact for fee
66. Oxidase and Catalase Tests for Pathogen Identification | Fee: Contact for fee
67. Protocol for Latex Agglutination Test for Pathogen Detection | Fee: Contact for fee
68. Host-Pathogen Interaction Assay Protocol | Fee: Contact for fee
69. Vaccine Preparation and Testing in Model Organisms | Fee: Contact for fee
70. Protocol for Serological Testing in Infectious Diseases | Fee: Contact for fee
71. Molecular Identification of Pathogens by PCR | Fee: Contact for fee
72. Protocol for Immunofluorescence Assay | Fee: Contact for fee
73. Preparation of Cell Culture Media for Pathogen Growth | Fee: Contact for fee
74. Procedure for Detecting Fungal Pathogens in Clinical Samples | Fee: Contact for fee
75. Protocol for Virus Cultivation in Embryonated Eggs | Fee: Contact for fee
76. Protocol for Lymphocyte Isolation from Blood Samples | Fee: Contact for fee
77. Flow Cytometry Protocol for Immune Cell Analysis | Fee: Contact for fee
78. ELISA Protocol for Detecting Host Antibodies | Fee: Contact for fee
79. Western Blot Protocol for Protein Detection in Immune Responses | Fee: Contact for fee
80. Protocol for Detecting Cytokines in Immune Response | Fee: Contact for fee
81. In Vitro Assay for T-Cell Activation | Fee: Contact for fee
82. In Vivo Assay for Measuring Immune Response to Vaccines | Fee: Contact for fee
83. Macrophage Phagocytosis Assay Protocol | Fee: Contact for fee
84. Protocol for Antigen-Antibody Agglutination Reactions | Fee: Contact for fee
85. Cell-Mediated Immunity Assay Protocol | Fee: Contact for fee
86. Protocol for Soil Microbiome Analysis for Crop Health | Fee: Contact for fee
87. Method for Isolation of Nitrogen-Fixing Bacteria | Fee: Contact for fee
88. Test for Plant Growth-Promoting Rhizobacteria (PGPR) | Fee: Contact for fee
89. Protocol for Biofertilizer Preparation Using Soil Microbes | Fee: Contact for fee
90. Production of Biopesticides Using Bacillus Thuringiensis | Fee: Contact for fee
91. Protocol for Plant-Microbe Interaction Studies | Fee: Contact for fee
92. Isolation of Endophytes from Plant Tissues | Fee: Contact for fee
93. Screening of Soil Microbes for Enzyme Production | Fee: Contact for fee
94. Protocol for Microbial Inoculant Production for Agriculture | Fee: Contact for fee
95. Test for Microbial Degradation of Agricultural Pesticides | Fee: Contact for fee
96. Protocol for Culturing Bacteriophages | Fee: Contact for fee
97. Plaque Assay for Quantification of Viruses | Fee: Contact for fee
98. Protocol for Viral RNA Extraction and Purification | Fee: Contact for fee
99. PCR and RT-PCR for Viral Genome Detection | Fee: Contact for fee
100. Protocol for Electron Microscopy Preparation of Viruses | Fee: Contact for fee
101. Viral Hemagglutination Assay Protocol | Fee: Contact for fee
102. Antiviral Drug Testing Protocol in Cell Cultures | Fee: Contact for fee
103. Procedure for Bacteriophage Therapy Assays | Fee: Contact for fee
104. Protocol for Vaccine Efficacy Testing in Virus Models | Fee: Contact for fee
105. Serological Testing Protocol for Viral Infections | Fee: Contact for fee

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