Microbiology Job Oriented Training

NTHRYS provides Microbiology Job Oriented Training for interested candidates at its Hyderabad facility, Telangana. Please refer below for more details including Fee strctures, Eligibility, Protocols and Modules etc.,. Please do call / message / whatsapp for more details on 9014935156 [India - +91]

Eligibility: BSc / BTech / MSc / MTech / MPhil / PhD in any Life Sciences studying or completed students

Protocols / Techniques Covered

Module I - Basic Microbiology

59 Protocols

1. Sterilization Techniques
   1. Autoclaving
      1. Operation of an Autoclave
      2. Autoclave Maintenance
   2. Dry Heat Sterilization
      1. Hot Air Oven Usage
   3. Filter Sterilization
      1. Use of Membrane Filters
      2. HEPA Filter Usage
   4. Chemical Sterilization
      1. Use of Ethanol, Bleach, and Other Disinfectants
2. Media Preparation
   1. Preparation of Nutrient Agar
      1. Recipe and Sterilization
   2. Preparation of Nutrient Broth
      1. Recipe and Sterilization
   3. Preparation of Selective and Differential Media
      1. MacConkey Agar
      2. Mannitol Salt Agar
   4. Preparation of Enrichment Media
      1. Selenite F Broth
      2. Tetrathionate Broth
3. Aseptic Technique
   1. Sterile Work Area Preparation
      1. Setting up a Laminar Flow Hood
   2. Flame Sterilization of Tools
      1. Sterilization of Inoculating Loops and Needles
   3. Aseptic Transfer Techniques
      1. Transferring Cultures Between Media
      2. Inoculating Slants and Stabs
4. Culturing Microorganisms
   1. Streak Plate Method
      1. Isolating Pure Cultures
   2. Spread Plate Method
      1. Quantifying Microbial Load
   3. Pour Plate Method
      1. Isolating Anaerobic Microorganisms
   4. Slant Culture Preparation
      1. Long-term Culture Maintenance
   5. Broth Culture Maintenance
      1. Sub-culturing Techniques
5. Microscopy
   1. Preparation of Wet Mounts
      1. Observing Live Cells
   2. Simple Staining
      1. Using Basic Dyes (e.g., Methylene Blue)
   3. Gram Staining
      1. Differential Staining Technique
   4. Acid-Fast Staining
      1. Ziehl-Neelsen Method
   5. Spore Staining
      1. Schaeffer-Fulton Method
   6. Capsule Staining
      1. Negative Staining Technique
6. Bacterial Growth Measurement
   1. Optical Density Measurement
      1. Using a Spectrophotometer
   2. Colony Counting
      1. Using a Hemocytometer
      2. Colony Forming Unit (CFU) Calculation
   3. Dry Weight Measurement
      1. Biomass Determination
7. Bacterial Identification
   1. Morphological Characterization
      1. Colony Morphology Analysis
   2. Biochemical Tests
      1. Catalase Test
      2. Oxidase Test
      3. Urease Test
      4. Nitrate Reduction Test
   3. IMViC Tests
      1. Indole Test
      2. Methyl Red Test
      3. Voges-Proskauer Test
      4. Citrate Utilization Test
   4. Sugar Fermentation Tests
      1. Triple Sugar Iron Agar Test
      2. Carbohydrate Fermentation Test
8. Enumeration of Bacteria
   1. Serial Dilution Method
      1. Plate Count Technique
   2. Most Probable Number (MPN) Method
      1. Estimation of Microbial Density
9. Antimicrobial Susceptibility Testing
   1. Kirby-Bauer Disk Diffusion Method
      1. Antibiotic Sensitivity Testing
   2. Minimum Inhibitory Concentration (MIC) Determination
      1. Broth Dilution Method
      2. E-test Method
10. Microbial Preservation
    1. Glycerol Stock Preparation
       1. Freezing Microbial Cultures
    2. Lyophilization (Freeze-Drying)
       1. Long-term Preservation
11. Motility Testing
    1. Hanging Drop Method
       1. Observing Live Motility
    2. Soft Agar Stabbing Method
       1. Testing for Motility in Semi-Solid Media
12. Enzyme Activity Assays
    1. Amylase Activity Test
       1. Starch Hydrolysis Test
    2. Protease Activity Test
       1. Casein Hydrolysis Test
    3. Lipase Activity Test
       1. Tributyrin Agar Test
    4. Gelatinase Test
       1. Gelatin Liquefaction
13. Anaerobic Cultivation
    1. Use of Anaerobic Jars
       1. Creating Anaerobic Conditions
    2. Use of Reducing Agents in Media
       1. Thioglycolate Broth
14. Additional Basic Techniques
    1. Growth Curve Analysis
       1. Monitoring Bacterial Growth Phases
    2. Plasmid Curing
       1. Removing Plasmids from Bacterial Cells
    3. Mutagenesis
       1. Inducing Mutations in Bacterial Cells
    4. Biofilm Formation Assays
       1. Studying Bacterial Biofilms
    5. Cold Enrichment
       1. Isolating Psychrophiles

Module - II - Industrial Microbiology

1. Quality Control (QC)
   1. Microbial Limit Testing
      1. Total Aerobic Microbial Count (TAMC)
      2. Total Yeast and Mold Count (TYMC)
      3. Detection of Specified Microorganisms (e.g., E. coli, Salmonella)
   2. Environmental Monitoring
      1. Air Sampling using Settle Plates
      2. Surface Sampling using Contact Plates
      3. Swab Sampling for Hard-to-Reach Areas
   3. Water Quality Testing
      1. Heterotrophic Plate Count (HPC)
      2. Endotoxin Testing using LAL Test
      3. Detection of Coliforms and E. coli using MPN Method
   4. Sterility Testing
      1. Membrane Filtration Method
      2. Direct Inoculation Method
   5. Antimicrobial Effectiveness Testing
      1. Preservative Efficacy Testing
      2. Challenge Tests
   6. Bioburden Testing
      1. Bioburden Determination
   7. Endotoxin Testing
      1. LAL (Limulus Amebocyte Lysate) Test
      2. Gel-Clot Method
      3. Chromogenic Method
2. Research and Development (R&D) - Microbial Strain and Process Development
   1. Strain Improvement
      1. Mutagenesis using Chemical Agents (e.g., EMS, NTG)
      2. UV Mutagenesis
      3. Recombinant DNA Technology for Strain Development
   2. Fermentation Process Development
      1. Shake Flask Studies for Initial Screening
      2. Bioreactor Optimization
      3. Media Optimization using Response Surface Methodology (RSM)
   3. Downstream Processing
      1. Cell Disruption
      2. Protein Extraction and Purification
      3. Semi and Ultra-filtration
3. Research and Development (R&D) - Analytical and Applied Microbiology
   1. Bioassays
      1. Enzyme Activity Assays (e.g., Amylase, Protease)
      2. Antibiotic Potency Testing using Microdilution Method
   2. Microbial Pathogenicity Testing
      1. Virulence Factor Assays
      2. Infection Models using Cell Cultures
   3. Biofilm Studies
      1. Biofilm Formation Assays
      2. Biofilm Disruption and Treatment Testing
   4. Bioremediation Research
      1. Isolation and Screening of Degrading Microorganisms
      2. Optimization of Degradation Conditions
   5. Microbial Ecology Studies
      1. Microbial Community Analysis using Metagenomics
      2. Functional Diversity Studies
4. Research and Development (R&D) - Industrial Bioproducts
   1. Biofuel Production
      1. Microbial Strain Selection for Biofuel Production
      2. Fermentation Process for Ethanol Production
      3. Biogas Production using Anaerobic Digestion
   2. Enzyme Production
      1. Microbial Production of Industrial Enzymes
      2. Enzyme Purification Techniques
      3. Stability and Activity Testing of Enzymes
   3. Biopolymer Production
      1. Production of Biopolymers
      2. Purification of Biopolymers
   4. Microbial Production of Vitamins and Amino Acids
      1. Fermentation Process for Vitamin Production
      2. Optimization of Microbial Strains for Amino Acid Production
5. Research and Development (R&D) - Bioprocess Engineering
   1. Bioreactor Design and Optimization
      1. Design of Laboratory-Scale Bioreactors
      2. Optimization of Bioreactor Parameters (e.g., pH, Temperature, Aeration)
   2. Process Monitoring and Control
      1. Real-Time Monitoring of Fermentation Parameters
      2. Use of Sensors and Control Systems in Bioprocesses
   3. Continuous Bioprocessing
      1. Design and Implementation of Continuous Fermentation Systems
      2. Advantages and Challenges of Continuous Bioprocessing
6. Production
   1. Continuous Fermentation
      1. Design and Setup of Continuous Fermentation Systems
      2. Monitoring and Control of Continuous Cultures
   2. Batch Fermentation
      1. Preparation and Inoculation of Fermenters
      2. Control of Fermentation Parameters (pH, Temperature, Aeration)
   3. Quality Control in Production
      1. In-Process Control: Monitoring Critical Parameters during Production
      2. Final Product Testing: Ensuring Product Purity and Potency
   4. Microbial Production of Pharmaceuticals
      1. Production of Antibiotics (e.g., Penicillin, Streptomycin)
      2. Production of Vaccines (e.g., Bacterial and Viral Vaccines)
   5. Microbial Production of Industrial Enzymes
      1. Production of Amylases, Proteases, and Lipases
      2. Enzyme Purification
   6. Microbial Production of Biofuels
      1. Ethanol Production using Yeast Fermentation
      2. Biogas Production using Anaerobic Digestion

1. Medical Microbiology
   1. Clinical Sample Collection
      1. Blood Culture Collection (Theory)
      2. Urine Sample Collection (Theory)
      3. Swab Collection for Throat and Wound Infections (Theory)
      4. Sputum Collection (Theory)
      5. Stool Sample Collection (Theory)
   2. Pathogen Isolation and Identification
      1. Streak Plate Method for Bacterial Isolation
      2. Spread Plate Method
      3. Pour Plate Method
      4. Biochemical Tests for Pathogen Identification
   3. Antimicrobial Susceptibility Testing
      1. Kirby-Bauer Disk Diffusion Method
      2. MIC Determination using Broth Dilution Method
      3. E-test Method
   4. Mycological Techniques
      1. Isolation and Identification of Fungi
      2. Fungal Staining Technique
      3. Yeast Identification using Chromogenic Media
      4. Antifungal Susceptibility Testing
   5. Virology (Bacteriophage) Techniques
      1. Viral Culture and Isolation
      2. Plaque Assay for Virus Quantification
      3. Hemagglutination Assay
      4. Virus Neutralization Test
2. Environmental Microbiology
   1. Ecosystem Microbiology
      1. Microbial Diversity Studies in Different Ecosystems
      2. Biogeochemical Cycling Studies
      3. Microbial Biomass Estimation in Ecosystems
   2. Bioremediation Microbiology
      1. Isolation of Pollutant-Degrading Microorganisms
      2. Microbial Degradation of Environmental Pollutants
      3. Bioaugmentation Techniques
      4. Biostimulation Techniques
   3. Extreme Environment Microbiology
      1. Isolation of Extremophiles from Extreme Environments
      2. Characterization of Extremophilic Microorganisms
      3. Thermophiles Isolation and Characterization
      4. Psychrophiles Isolation and Characterization
   4. Biofilm Studies
      1. Biofilm Formation Assays
      2. Biofilm Disruption and Treatment Testing
      3. Microscopic Analysis of Biofilms
      4. Biofilm Quantification using Crystal Violet Staining
   5. Biodegradation Studies
      1. Isolation of Waste-Degrading Microorganisms
      2. Assessment of Degradation Efficiency
      3. Biodegradation Kinetics Studies
      4. Microbial Consortia for Biodegradation
3. Agricultural Microbiology
   1. Plant-Microbe Interaction Studies
      1. Isolation of Rhizobia from Root Nodules
      2. Assay for Nitrogen Fixation
      3. Phosphate Solubilization Assay
      4. Isolation of Plant Growth-Promoting Rhizobacteria (PGPR)
   2. Soil Health Assessment
      1. Microbial Biomass Carbon Measurement
      2. Enumeration of Soil Microorganisms
      3. Soil Respiration Rate Measurement
      4. Soil Enzyme Activity Assays
   3. Biological Control Agents
      1. Isolation of Antagonistic Microorganisms
      2. Biocontrol Efficacy Testing
      3. Production of Biocontrol Formulations
      4. Field Trials for Biocontrol Agents
   4. Composting Microbiology
      1. Isolation of Composting Microorganisms
      2. Monitoring of Compost Microbial Community
      3. Optimization of Composting Conditions
      4. Quality Assessment of Compost
   5. Microbial Fertilizers
      1. Production of Biofertilizers
      2. Quality Control of Biofertilizers
      3. Application of Biofertilizers
      4. Impact Assessment of Biofertilizers on Crop Yield
4. Textile Microbiology
   1. Microbial Deterioration of Textiles
      1. Isolation of Textile-Degrading Microorganisms
      2. Assessment of Fungal and Bacterial Damage to Textiles
      3. Quantification of Microbial Load on Textiles
      4. Identification of Microbial Species Involved in Deterioration
   2. Biotreatment of Textiles
      1. Enzymatic Desizing
      2. Biopolishing and Biostoning of Fabrics
      3. Microbial Degumming of Silk
      4. Biofinishing of Wool
   3. Antimicrobial Textiles
      1. Production of Antimicrobial Fabrics
      2. Testing Antimicrobial Efficacy of Treated Textiles
      3. Assessment of Durability of Antimicrobial Treatments
      4. Microbial Resistance Testing on Treated Textiles
5. Leather Microbiology
   1. Microbial Deterioration of Leather
      1. Isolation of Leather-Degrading Microorganisms
      2. Assessment of Microbial Damage to Leather
      3. Identification of Fungal Species on Leather
      4. Quantification of Bacterial Load on Leather
   2. Biotreatment of Leather
      1. Enzymatic Unhairing
      2. Biotanning using Microbial Enzymes
      3. Enzymatic Degreasing
      4. Microbial Dehairing Process Optimization
   3. Leather Preservation
      1. Use of Antimicrobial Agents for Leather Preservation
      2. Testing of Preservatives for Efficacy and Safety
      3. Microbial Load Monitoring during Leather Storage
      4. Prevention of Biofilm Formation on Leather
6. Water Microbiology
   1. Drinking Water Testing
      1. Microbial Quality Analysis using MPN Method
      2. Detection of Pathogenic Bacteria
      3. Detection of Enteric Viruses
      4. Assessment of Protozoan Parasites
   2. Recreational Water Testing
      1. Isolation and Enumeration of Indicator Organisms
      2. Assessment of Waterborne Pathogens
      3. Quantification of Fecal Coliforms
      4. Detection of Harmful Algal Blooms
   3. Industrial Water Testing
      1. Microbial Analysis of Cooling Tower Water
      2. Detection of Legionella spp.
      3. Microbial Corrosion Monitoring
      4. Biofouling Assessment
   4. Wastewater Testing
      1. Microbial Analysis of Wastewater
      2. BOD and COD Measurement
      3. Detection of Pharmaceutical Residues
      4. Quantification of Microbial Contaminants
7. Air Microbiology
   1. Indoor Air Quality Testing
      1. Air Sampling using Impactors
      2. Quantification of Airborne Fungi and Bacteria
      3. Assessment of Indoor Airborne Pathogens
      4. Identification of Mold Spores in Indoor Air
   2. Outdoor Air Quality Testing
      1. Monitoring of Airborne Pollutants
      2. Identification of Bioaerosols
      3. Quantification of Particulate Matter
      4. Detection of Pollen Grains in Air
   3. Occupational Air Quality Monitoring
      1. Air Sampling in Industrial Settings
      2. Assessment of Microbial Load in Work Environments
      3. Testing for Occupational Allergens
      4. Monitoring for Bioaerosol Exposure
   4. Airborne Disease Transmission Studies
      1. Sampling and Detection of Airborne Pathogens
      2. Assessment of Airborne Transmission Risks
      3. Evaluation of Air Filtration Systems
      4. Studies on Pathogen Survival in Air
8. Soil Microbiology
   1. Soil Health Indicators
      1. Microbial Biomass Carbon and Nitrogen
      2. Enzyme Activity Assays (e.g., Dehydrogenase, Phosphatase)
      3. Soil Respiration Measurement
      4. Assessment of Soil Organic Matter
   2. Plant Growth-Promoting Rhizobacteria (PGPR)
      1. Isolation and Characterization of PGPR
      2. Screening for Plant Growth-Promoting Traits
      3. Greenhouse Trials with PGPR
      4. Field Trials with PGPR
   3. Soil Microbial Interactions
      1. Study of Soil Microbial Food Webs
      2. Microbial Interactions with Soil Fauna
      3. Microbial Influence on Soil Structure
      4. Microbial Impact on Soil Nutrient Cycling
   4. Bioremediation of Contaminated Soils
      1. Isolation of Pollutant-Degrading Microorganisms
      2. Assessment of Bioremediation Efficiency
      3. Bioaugmentation Techniques
      4. Phytoremediation Studies
9. Waste Microbiology
   1. Biodegradation Studies
      1. Isolation of Waste-Degrading Microorganisms
      2. Assessment of Degradation Efficiency
      3. Biodegradation Kinetics Studies
      4. Microbial Consortia for Biodegradation
   2. Composting Microbiology
      1. Isolation of Composting Microorganisms
      2. Monitoring of Compost Microbial Community
      3. Optimization of Composting Conditions
      4. Quality Assessment of Compost
   3. Landfill Microbiology
      1. Microbial Analysis of Landfill Sites
      2. Detection of Methanogenic Microorganisms
      3. Biogas Production Studies
      4. Assessment of Microbial Contamination in Landfills
   4. Waste Treatment Microbiology
      1. Isolation of Microorganisms for Wastewater Treatment
      2. Assessment of Wastewater Treatment Efficiency
      3. Bioreactor Studies for Waste Treatment
      4. Microbial Analysis of Effluent Quality
   5. Bioplastic Degradation Studies
      1. Isolation of Bioplastic-Degrading Microorganisms
      2. Assessment of Bioplastic Degradation
      3. Enzymatic Analysis of Bioplastic Degradation
      4. Environmental Impact of Bioplastic Degradation
10. Sewage Microbiology
    1. Sewage Treatment Plant Monitoring
       1. Microbial Analysis of Influent and Effluent
       2. Determination of BOD and COD Levels
       3. Microbial Community Analysis in Treatment Systems
       4. Optimization of Treatment Processes
    2. Pathogen Detection in Sewage
       1. Isolation of Enteric Pathogens
       2. Quantification of Pathogen Load
       3. Detection of Viral Pathogens
       4. Assessment of Protozoan Contaminants
    3. Sludge Treatment Microbiology
       1. Microbial Analysis of Sewage Sludge
       2. Composting of Sewage Sludge
       3. Assessment of Pathogen Reduction in Sludge
       4. Biogas Production from Sludge
    4. Antibiotic Resistance in Sewage
       1. Detection of Antibiotic-Resistant Bacteria
       2. Assessment of Antibiotic Resistance Genes
       3. Impact of Sewage Treatment on Resistance Genes
       4. Monitoring of Resistance Gene Dissemination
11. Marine Microbiology
    1. Marine Water Quality Testing
       1. Isolation of Marine Microorganisms
       2. Assessment of Microbial Pollution in Marine Waters
       3. Detection of Harmful Algal Blooms
       4. Quantification of Fecal Indicator Bacteria
    2. Marine Sediment Analysis
       1. Microbial Community Analysis in Sediments
       2. Detection of Biodegradative Microorganisms
       3. Isolation of Anaerobic Marine Microorganisms
       4. Assessment of Sediment Microbial Activity
    3. Marine Biotechnology
       1. Extraction of Bioactive Compounds from Marine Microbes
       2. Production of Marine-Derived Enzymes
       3. Marine Microorganisms in Bioremediation
       4. Marine Natural Product Screening
    4. Microbial Symbiosis in Marine Organisms
       1. Study of Symbiotic Relationships in Marine Organisms
       2. Isolation of Symbiotic Microorganisms
       3. Characterization of Symbiotic Interactions
       4. Impact of Symbiosis on Host Health
    5. Marine Biogeochemistry
       1. Role of Microorganisms in Marine Biogeochemical Cycles
       2. Microbial Mediation of Carbon and Nitrogen Cycles
       3. Study of Microbial Loop in Marine Ecosystems
       4. Microbial Influence on Marine Sediment Chemistry