Immunohistochemistry Training Program

Introduction to Immunohistochemistry

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• Fundamentals of antigen-antibody interactions in IHC
• Selection of primary and secondary antibodies for IHC
• Types of detection systems: chromogenic vs. fluorescent
• Troubleshooting common issues in IHC experiments

IHC Staining Techniques

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• Paraffin embedding and microtomy for sectioning
• Immunostaining protocols for single and multiplex IHC
• Optimization of antigen retrieval methods
• Counterstaining techniques and visualization strategies

Applications in Research and Clinical Diagnosis

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• Application of IHC in neurodegenerative disease studies
• Role of IHC in infectious disease diagnostics
• Standardization and validation of IHC assays
• Comparison of IHC with other histological techniques

Individual Protocols Under Immunohistochemistry Training Program

1. Overview of immunohistochemistry and its applications | Fee: Contact for fee
2. Fundamentals of antigen-antibody interactions in IHC | Fee: Contact for fee
3. Selection of primary and secondary antibodies for IHC | Fee: Contact for fee
4. Types of detection systems: chromogenic vs. fluorescent | Fee: Contact for fee
5. Troubleshooting common issues in IHC experiments | Fee: Contact for fee
6. Fixation and processing of tissue samples for IHC | Fee: Contact for fee
7. Paraffin embedding and microtomy for sectioning | Fee: Contact for fee
8. Immunostaining protocols for single and multiplex IHC | Fee: Contact for fee
9. Optimization of antigen retrieval methods | Fee: Contact for fee
10. Counterstaining techniques and visualization strategies | Fee: Contact for fee
11. Using IHC for cancer biomarker detection | Fee: Contact for fee
12. Application of IHC in neurodegenerative disease studies | Fee: Contact for fee
13. Role of IHC in infectious disease diagnostics | Fee: Contact for fee
14. Standardization and validation of IHC assays | Fee: Contact for fee
15. Comparison of IHC with other histological techniques | Fee: Contact for fee

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